Proteoglycan Protocols by Renato V. Iozzo, Renato V. Iozzo (Editor), Renato V. Lozzo (Editor)

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(Hardcover)

  • Pub. Date: October 2007
  • 576pp

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    Product Details

    • Pub. Date: October 2007
    • Publisher: Springer-Verlag New York, LLC
    • Format: Hardcover, 576pp

    Synopsis

    Renato Iozzo and an authoritative team of investigators present for the first time a comprehensive and up-to-date collection of readily reproducible preparative and analytical methods for the in-depth analysis of these important compounds. Featuring step-by-step protocols, this book will enable both novice and experienced researchers to isolate intact proteoglycans from tissues and cultured cells, to establish the composition of their carbohydrate moieties, and to generate strategies for prokaryotic and eukaryotic expression. There are also detailed techniques for the suppression of specific proteoglycan genes, for the detection of mutant cells and their degradation products, and for studying specific interactions between proteoglycans and extracellular matrix proteins and with growth factors and their receptors.

    Booknews

    Contains 51 papers presenting readily reproducible preparative and analytical methods for the detailed analysis of proteoglycans, highly complex macromolecules found in mammalian and lower organisms. Suitable for both novice and experienced researchers, the protocols guide readers through the process of isolating intact proteoglycans from tissues and cultured cells, establishing the composition of their carbohydrate moieties, and generating strategies for prokaryotic and eukaryotic expression. Also presented are detailed techniques for the suppression of specific proteoglycan genes, for the detection of mutant cells and their degradation products, and for studying specific interactions between proteoglycans and extracellular matrix proteins and with growth factors and their receptors. Each section includes a general introduction, a detailed materials section, and a step-by-step methods section, accompanied by extensive notation including troubleshooting tips and practical considerations. Written by and of likely interest to molecular biologists, cell biologists, pathologists, biochemists, geneticists, oncologists, hematologists, rheumatologists, infectious disease specialists, and pediatricians. Annotation c. Book News, Inc., Portland, OR (booknews.com)

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    Biography

    Iozzo, Renato V., MD

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