Synopsis

Professor Michael N. Marsh, a recognized world authority on this condition, together with a team of other expert laboratory/clinical investigators from around the world, present a collection of the state-of-the-art techniques for studying the biology and immunopathology of celiac disease. Both the novice and experienced researcher will find in this collection detailed step-by-step methods for cloning lymphocytes, creating gene/peptide libraries, and performing genotyping, linkage, and positional cloning. Also included are techniques for determining the peptide structure of HLA-bound material (tandem mass spectroscopy), computerized morphometry, in situ hybridization, organ culture, and monoclonal AB assays.
Comprehensive and cutting-edge, Celiac Disease: Methods and Prools provides all experimental and clinical investigators with the essential core of readily reproducible methods necessary for successful work on celiac disease today.

Booknews

Research gastroenterologists, immunologists, and other scientists detail laboratory methods for studying the biology and immunopathology of celiac disease, which involves sensitivity to gluten. They include reproducible techniques for cloning lymphocytes, using physical methods to identify gluten sequences as T-cell antigens, studying peptide responses and by either jejunal or rectal challenge, elucidating the locations of other genes concerned in pathogenesis, and using elegant immuno-histo-cyto-chemical and mRNA probing techniques for analyzing the finer points of mucosal inflammatory response to gluten. Gluten sensitivity research is gaining popularity, and the descriptions here should allow newcomers to begin with the current methods. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Biography

Marsh, Michael N., MD, DSc, FRCP (Univ of Manchester)